Product Details

CasNo： 10526-80-4

MF： C6H13N.C3H5O6P

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 99%

Basic Information

Product Description

Product Name：Phosphoenolpyruvic acid cyclohexylammonium salt

CAS No: 10526-80-4

Form: Powder

Product Application

1.Pyruvate Kinase (PK) Activity Assay (Core Application):

Serves as the direct substrate​ for PK assays. Essential in clinical diagnostics and research for measuring PK activity in serum, cell, or tissue lysates. A key tool for studying glycolytic flux, erythrocyte disorders (e.g., pyruvate kinase deficiency), and cancer metabolism (Warburg effect).

2.ATP Regeneration Systems:

Used in conjunction with Pyruvate Kinase (PK)​ and ADP​ to form an efficient ATP regeneration cycle (PEP + ADP → (PK) → ATP + Pyruvate) for ATP-consuming enzymatic reactions (e.g., protein phosphorylation, DNA/RNA synthesis). This system maintains a constant ATP concentration, driving reactions forward and conserving expensive ATP.

3.Metabolic Pathway Studies:

Used for in vitroreconstitution and regulation studies of central metabolic pathways like glycolysis and gluconeogenesis.

Serves as a precise phosphoryl group donor for studying phosphotransferases.

4.Diagnostic Kit Component:

A key ingredient in commercial "Serum Pyruvate Kinase Assay" kits.

Packaging

25Kg/Drum

Storage

Store at -20°C in a tightly sealed container, desiccated, and protected from light.​ Aliquotting after opening is advised to avoid repeated freeze-thaw cycles and moisture uptake.