English Name: Phosphoenolpyruvic acid cyclohexylammonium salt
CAS No.: 10526-80-4
Molecular Formula: C3H4O6P⋅C6H14N
Molecular Weight: 267.22
Physicochemical Properties
Appearance: White to off - white powder.
Solubility: Easily soluble in water, with a solubility of 100mg/ml in water.
Melting Point: 148 - 150℃ (decomposition).
Stability: Sensitive to heat, should be stored at - 20℃ in a cool and dry place, protected from light.
Technical Indicators
Purity: Usually ≥97% (enzymatic method), and there are also products with a purity of 98%.
Uses
Biochemical Research: Phosphoenolpyruvic acid (PEP) is an important biochemical molecule in biological cells, containing a high - energy phosphate bond (-61.9 kJ/mol), and is involved in the processes of glycolysis and gluconeogenesis. In glycolysis, PEP is formed from 2 - phosphoglycerate by the action of enolase, and then converted into pyruvate by the catalysis of pyruvate kinase, while generating 1 molecule of ATP through substrate - level phosphorylation. In gluconeogenesis, PEP is formed from the decarboxylation of oxaloacetate and the hydrolysis of 1 molecule of GTP, and this reaction is catalyzed by phosphoenolpyruvate carboxykinase, which is the rate - limiting step in gluconeogenesis.
Plant Physiological Research: In plants, PEP can be used to synthesize chorismate through the shikimate pathway, and then be metabolized into aromatic amino acids (phenylalanine, tryptophan, and tyrosine) and other aromatic compounds. In addition, in C4 plants, PEP is an important substrate for carbon fixation. Under the catalysis of phosphoenolpyruvate carboxylase, PEP reacts with CO₂ to form oxaloacetate.