Proteinase K

Product Details

CasNo： 39450-01-6

MF： C29H27N2O12P

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 90%

• Basic Information
  • English Name: Proteinase K
  • Source: Originally isolated from Tritirachium album, and now it is mostly expressed in host cells such as Pichia pastoris through genetic engineering technology.
  • CAS Number: 39450 - 01 - 6
• Product Properties
  • Appearance: Usually white to off - white amorphous powder or lyophilized powder.
• Specifications and Packaging
  • Specifications: Common enzyme activity unit specifications include ≥30U/mg, ≥40U/mg, etc. There are also specifications in the form of solutions, such as 20mg/ml.
  • Packaging: There are small - scale packages for experimental research, such as 0.2ml, 1ml, 5ml liquid packages, and 25mg, 100mg lyophilized powder packages. Larger - scale custom packaging is also available according to customer requirements.
• Activity and Related Parameters
  • Activity Definition: One unit is defined as the enzyme activity that produces 1 μmol of tyrosine per minute from casein at 37 °C and pH 7.5.
  • Optimal pH: It is active in a wide pH range of 4.0 - 12.5, with the optimal pH range being 7.5 - 12.0.
  • Optimal Temperature: The optimal reaction temperature is between 20 - 60 °C.
  • Molecular Weight: Approximately 27kDa.
• Stability
  • Storage Stability: The dry powder is stable for at least 2 years at - 20 °C, and there is no obvious loss of activity after 10 freeze - thaw cycles.
  • Stability after Dissolution: The dissolved Proteinase K solution can be stored stably at - 15 to - 25 °C, and the specific storage time varies according to different products.
• Quality Parameters
  • Purity: For example, the Proteinase K product of Qiagen has a protein content of >70%, and the DNA content of the Ultra pure product is ≤0.1pg/mg.
• Application Fields
  • Nucleic Acid Extraction: It can effectively digest proteins, including DNases and RNases. During nucleic acid extraction, it can quickly inactivate endogenous nucleases to ensure the integrity of the extracted DNA or RNA. It is especially suitable for isolating natural RNA and DNA from tissues or cells.
  • Protein Research: It is used to digest protein samples. For example, in experiments such as protein sequencing and protein structure analysis, it helps to remove impurity proteins or break down proteins into polypeptide fragments.
  • Biopharmaceuticals: In the process of vaccine production and other fields, it is used to remove miscellaneous proteins and improve product purity.