Product Details
CasNo： 9025-24-5
Appearance： powder
Delivery Time： 15 days
Packing： 25kg/drum
Purity： 90%
- Basic Information
- Chinese Name: Carboxypeptidase B
- English Name: Carboxypeptidase B, abbreviated as CPB
- EC Number: 3.4.17.2
- Source: Recombinant carboxypeptidase B is expressed in Pichia pastoris through genetic engineering technology, and the natural one is mainly derived from animal tissues such as porcine pancreas.
- Product Properties
- Appearance: Usually white or off - white freeze - dried powder.
- Specifications and Packaging
- Specifications: Common specifications are enzyme activity units, such as ≥150U/mg, ≥170U/mg, etc. There are also specifications in the form of liquid, such as 1mL (containing a certain amount of enzyme protein).
- Packaging: There are small - scale packages for experimental research, such as 1mL, 5mL liquid packages, and milligram - scale and gram - scale freeze - dried powder packages. It can also be customized according to customer needs.
- Activity and Related Parameters
- Activity Definition: Under the conditions of 37°C and pH 7.6, the amount of enzyme that catalyzes the hydrolysis of 1μmol of hippuryl - L - arginine per minute is defined as one enzyme activity unit (U).
- Optimal pH: Generally between 7.5 - 8.5. Both acidic and alkaline conditions will cause a decrease in activity. When the pH is close to 5.0, the activity is close to zero, and when the pH>12.0, the activity is completely lost.
- Optimal Temperature: Usually 37°C. Within a certain temperature range (<60°C), the enzyme digestion activity can increase with the increase of temperature.
- Molecular Weight: Approximately 35kDa. For example, the recombinant carboxypeptidase B of Chongqing Imeddy Biotechnology Co., Ltd. has a molecular weight of 35kDa, and the recombinant carboxypeptidase B of Sigma - Aldrich has a molecular weight of 34.6kDa.
- Stability
- Storage Stability: The dry powder is stable for 24 months when stored at - 15°C or below.
- Stability after Dissolution: After being configured and dissolved, it is stable for 3 times of repeated freezing and thawing when stored at - 15°C or below. It can be stored at 2 - 8°C for 3 days after dissolution.
- Quality Parameters
- Purity: ≥95% detected by SDS - PAGE.
- Endotoxin: <10EU/mg.
- Application Fields
- Biopharmaceuticals: In the production process of recombinant insulin and its analogs or recombinant polypeptides, it is used to remove the C - terminal basic amino acids.
- Protein Research: It can be used for protein sequence analysis. The relevant sequence information can be obtained by continuously cleaving the C - terminal basic amino acids from the protein.
- Production and Source
- Genetic Engineering Technology: The gene encoding carboxypeptidase B is introduced into a suitable host cell, such as Pichia pastoris. Through fermentation culture, separation and purification and other processes, recombinant carboxypeptidase B is obtained. This is the currently commonly used production method, which has the advantages of high yield, high purity and low cost.
In addition, the product information of some other companies is as follows:
- Laurus Bioscience's Rc Carboxypeptidase B
- Molecular Weight: 34.5kDa.
- Amino Acid Composition: Consisting of 307 amino acids.
- Purity: ≥90% (SDS - PAGE).
- Specific Activity: >170 units/mg of protein (hippuryl - L - arginine).
- Product Form: Colorless, white to off - white clear liquid or white to off - white odorless powder.
- pH Value: 7.0 - 8.9.
- Protein Content: 2.5 - 3.5mg/ml (liquid), not less than 0.35mg/mg of powder (dry powder).
- Sigma - Aldrich's Recombinant Carboxypeptidase B
- Molecular Weight: 34.6kDa.
- Specific Activity: Approximately 150 units/mg protein (at 25°C with hippuryl - L - arginine as the substrate).
- Optimal pH: 7.0 - 9.0.
- Contaminants: Carboxypeptidase A≤2%, trypsin (using chymotrypsin as the substrate)≤0.005U/mg.
