Product Details
CasNo： 9028-53-9
Appearance： powder
Delivery Time： 15 days
Packing： 25kg/drum
Purity： 99%
Basic Information
- Chinese Name: Glucose Dehydrogenase
- English Name: Glucose Dehydrogenase, abbreviated as GDH
- CAS Number: 9028 - 53 - 9
- EINECS Number: 232 - 836 - 9
- Appearance: Usually white amorphous powder, and there are also purple amorphous powders, etc. For example, Toyobo's glucose dehydrogenase (pqq - dependent) is purple amorphous powder.
Specifications and Packaging
- Specifications: Common ones include enzyme activity unit specifications, such as ≥40U/mg, ≥300U/mg, 250U/mg, etc.; there are also specifications in the form of kits, such as 60T/50S, 48T/96T, etc.
- Packaging: There are large - package forms of 25kg/drum (customizable), and small - package forms for experimental research, etc.
Activity and Related Parameters
- Activity Definition: The activity definition of different products varies slightly. For example, the sbsgenetech company defines it as the amount of enzyme that converts 1 micromole of 2,6 - dichlorophenolindophenol (DCPIP) per minute at pH6.5 and 37°C as one enzyme activity unit; it can also be defined as the amount of enzyme that oxidizes 1.0 micromole of β - D - glucose to D - glucono - δ - lactone or D - galactose to D - galacturonic acid per minute at pH7.0 and 37°C as one unit.
- Michaelis Constant: For example, for the product of Toyobo company, its Michaelis constant (D - glucose) is 4.8 mM.
- Optimal pH: Generally between 7.0 - 8.0. Glucose dehydrogenases from different sources may vary. For example, the NAD - dependent glucose dehydrogenase from Bacillus has an optimal pH of 8.0, while the FAD - dependent one has an optimal pH of 7.0 - 8.0.
- Optimal Temperature: Usually between 37°C - 45°C. For example, the product of Toyobo company has an optimal temperature of 37°C, and the glucose dehydrogenase used for the production of fructooligosaccharides has an optimal temperature of 35°C - 45°C.
Stability
- Thermal Stability: The NAD - dependent glucose dehydrogenase is stable at 80°C and below when the pH is 7.0; the FAD - dependent glucose dehydrogenase is stable at 50°C and below when the pH is 6.0. Generally, glucose dehydrogenase is more stable at low temperatures, such as - 20°C for a long - term storage.
- pH Stability: For example, the PQQ - dependent glucose dehydrogenase is stable at pH3.5 - 8.5 (25°C, 16 hours); the FAD - dependent glucose dehydrogenase is stable at pH5.0 - 7.5 (25°C, 20 hours).
Application Fields
- Clinical Diagnosis: It is used for blood glucose detection and is a diagnostic enzyme for clinical blood glucose determination. It can detect the blood glucose concentration by catalyzing the glucose reaction and using the generated products.
- Biosensors: It can be used to construct glucose biosensors for detecting glucose content in environmental or biological samples.
- Food Industry: As a key enzyme for the preparation of high - content fructooligosaccharides, it can improve the yield and quality of fructooligosaccharides.
- Pharmaceutical Industry: It is involved in the synthesis of some drug intermediates and can also be used to study the disease mechanisms related to glucose metabolism.
Production and Source
- Microbial Fermentation: Glucose dehydrogenase is produced by culturing specific microbial strains, such as Bacillus, Aspergillus, etc., and then through separation, purification and other processes.
- Genetic Engineering Technology: Using genetic engineering means, the gene encoding glucose dehydrogenase is introduced into suitable host cells, such as Escherichia coli, Saccharomyces cerevisiae, etc., to make it efficiently express glucose dehydrogenase.
Information about Detection Kits
- Detection Principle: Glucose dehydrogenase can catalyze D - glucose and NAD(P) to generate D - gluconic acid and NAD(P)H. NADH has a characteristic absorption peak at 340nm, and the activity of glucose dehydrogenase can be characterized by the change of absorbance value.
- Kit Composition: Generally, it includes extraction solution, reagent 1, reagent 2, etc. Some kits also have reagent 3. The extraction solution is used for the extraction of glucose dehydrogenase in the sample, and reagent 1, reagent 2 and reagent 3 are used for the construction and detection of the reaction system.
Here are some product details of glucose dehydrogenase from different companies:
Glucose dehydrogenase from Pseudomonas sp. (Sigma - Aldrich)
- Appearance: White powder
- Activity: ≥200 U/mg
- Activity Definition: One unit corresponds to the amount of enzyme which will oxidize 1 μ mole β - D - glucose to D - glucono - δ - lactone per minute at pH 8.0 and 37 °C.
Glucose Dehydrogenase NAD - Dependent (Sekisui Diagnostics)
- Origin: Bacillus sp.
- Appearance: White powder
- Activity: >30 U/mg
- Contaminants: Lactate dehydrogenase ≤ 0.002%; NADH oxidase ≤ 0.002%
- Optimal pH: 8.0
- pH Stability: 5.0 - 8.0 (40 °C, 90 mins)
- Thermal Stability: Stable at 80 °C and below (pH 7.0, 50 mins)
Glucose Dehydrogenase FAD - GDH (Sekisui Diagnostics)
- Origin: Recombinant Aspergillus sojae
- Appearance: Yellow lyophilizate
- Activity: ≥475 U/mg lyophilizate
- Contaminants: NAD glucose dehydrogenase < 1.0×10⁻²%; Hexokinase < 1.0×10⁻²%; α - glucosidase < 1.0×10⁻²%; β - glucosidase < 1.0×10⁻²%
- Optimal pH: 7.0 - 7.5
- pH Stability: 2.5 - 7.5
- Optimal Temperature: 40 - 50 °C
- Thermal Stability: Below 50 °C
Glucose Dehydrogenase ex. Microorganism (SRL Chem)
- Activity: 250 U/mg powder
- Activity Definition: One unit will oxidize 1.0 micromole per minute of ß - D - glucose to D - glucono - d - lactone or D - galactose to D - galactonate at pH 7.0 at 37 °C in the presence of NADP⁺.
