Product Details
CasNo： 9030-66-4
Appearance： powder
Delivery Time： 15 days
Packing： 25kg/drum
Purity： 90%
Basic Information
- Chinese Name: Glycerol Kinase
- English Name: Glycerokinase, abbreviated as GK
- CAS Number: 9030 - 66 - 4
- Enzyme Commission Number: EC 2.7.1.30
Source
Glycerokinase can be derived from various microorganisms, such as Cellulomonas sp., Escherichia coli, etc.
Appearance
It is usually white amorphous powder or lyophilized powder, and there are also cases of brown to light yellow powder.
Molecular Characteristics
- Molecular Weight: The molecular weight of glycerokinase varies depending on the source. For example, the molecular weight of glycerokinase from Cellulomonas sp. is about 128kDa, while that from some microorganisms is about 220kDa.
- Isoelectric Point: Generally between 4.2 - 4.3.
- Molecular Structure: Some glycerokinases are composed of four subunits.
Activity and Unit Definition
- Activity: The activity unit of glycerokinase is usually defined as the amount of enzyme required to convert 1.0 micromole of glycerol and ATP to L - α - glycerophosphate and ADP per minute under specific conditions, which is one unit.
- Activity Range: Different products have different activity levels. For example, the activity of GYK - 311 is Grade Ⅲ, 30u/mg - solid or higher.
Main Functions
- Catalytic Reaction: It catalyzes the MgATP - dependent phosphorylation of glycerol to produce sn - glycerol - 3 - phosphate, which is the rate - limiting enzyme in glycerol utilization.
- Clinical Application: It is combined with glycerol - 3 - phosphate dehydrogenase, glycerol - 3 - phosphate oxidase, pyruvate kinase, lactate dehydrogenase, lipoprotein lipase, etc. for the enzymatic determination of glycerol and triglycerides in clinical analysis.
Relevant Characteristics
- Substrate Specificity: It has the highest specificity for glycerol, and can also phosphorylate dihydroxyacetone and glyceraldehyde.
- Inhibitors: Including p - chloromercuribenzoate, heavy metal ions (such as Pb²⁺, Fe²⁺, Hg²⁺, Ag⁺), etc.
- Optimal pH: 9.8 in the G - 3 - PDH system and 7.8 in the G - 3 - P oxidase system.
- Optimal Temperature: Generally about 50℃. However, for glycerokinase from some microorganisms, the optimal temperature can be 70℃.
- pH Stability: Stable in the range of pH 5.5 - 10.0.
- Thermal Stability: Stable below 40℃ (pH 7.5, 15min). For some glycerokinase products, it is stable below 65℃ (pH 7.5, 30min).
Storage Conditions
It generally needs to be stored at - 20℃, and it is stable for at least 1 year under this condition.
