Pyruvate Kinase

Product Details

CasNo： 9001 - 59 - 6

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 98%

• Basic Information
  • CAS Number: 9001 - 59 - 6
  • English Name: Pyruvate Kinase
  • Appearance: Usually presented as a white suspension.
  • Molecular Weight: The molar mass of pyruvate kinase is approximately 237,000, existing as a tetramer. Each polypeptide chain of the tetramer has a molar mass of 57,200.
  • Isoenzyme Forms: In mammals, there are four isoenzymes of pyruvate kinase, namely pyruvate kinase M1 (PKM1), pyruvate kinase M2 (PKM2), L - type pyruvate kinase (PKL), and R - type pyruvate kinase (PKR).
• Physical and Chemical Properties
  • Enzyme Activity: The specific activity of products from different manufacturers varies. For example, the pyruvate kinase products of Beijing Deaoping Biotechnology Co., Ltd. have a specific activity of ≥250U/mg for PK and PK01, and ≥200U/mg for PK02. The pyruvate kinase products of Shanghai Yuanye Biotechnology Co., Ltd. have a specific activity of ≥200U/mg. For the product from rabbit muscle of Roche, the specific activity is about 200 units/mg protein at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.
  • Substrate Specificity: It catalyzes the reaction of phosphoenolpyruvate and ADP to produce ATP and pyruvate.
  • Cofactors: It requires divalent metal ions (such as Mg²⁺ and Mn²⁺) and also depends on monovalent metal ions (such as K⁺, Rb⁺, Cs⁺).
  • Allosteric Effect: Pyruvate kinase is an allosteric enzyme, and its activity is regulated by allosteric activators and inhibitors. The main allosteric activators include FBP, serine, etc., and ATP, oxalic acid, alanine, etc. can be used as allosteric inhibitors of PKM2.
• Storage Conditions: Generally, it should be stored at - 20 °C. Some products, such as PK and PK01 of Deaoping, can also be stored at 4 °C for 24 months.
• Applications
  • Scientific Research Field: Pyruvate kinase can be used as a potential biomarker for meat color and tenderness. Its M2 subtype also has non - metabolic functions, which can regulate tumor cell metastasis, gene expression, etc. In addition, PK can be used to study the glycolytic process and related metabolic pathways.
  • Clinical Diagnosis: When myocardial cells are damaged, pyruvate kinase will be released into the blood, and its detection can be used to determine whether there is a myocardial infarction. At the same time, the abnormal activity of pyruvate kinase in red blood cells may lead to diseases such as anemia, and the detection of its activity is helpful for the diagnosis of related diseases.