GLDH

Product Details

CasNo： 9029-12-3

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 99%

1. Basic Information​

• CAS Number: 9029 - 12 - 3​

• Common Name: L - Glutamic Dehydrogenase Type IV (from Bovine Liver) [This is a likely common name based on the information available, as some sources suggest it is related to this enzyme from bovine liver]​

• Appearance:​

• Can be presented in different forms. For example, it might be a viscous solution with an amber color when in liquid form. In solid form, it could be a lyophilized powder.​

• Molecular Formula: C₂₄H₄₆N₂O​

• Molecular Weight: 378.60​

2. Physical and Chemical Properties​

• Enzyme Activity:​

• When sourced from bovine liver and in certain preparations like the type I ammonium sulfate suspension, it has a specific activity of ≥ 40 units/mg protein. For the type III lyophilized powder from bovine liver, the specific activity is ≥ 20 units/mg protein. If sourced from E. coli cell culture, it has a tested activity of not less than 80 U/mg.​

• Storage Conditions: Recommended to be stored at - 20 °C. This low - temperature storage helps to maintain the enzyme's stability and activity over time, preventing denaturation and degradation.​

• Solubility and State: As mentioned, it can exist as a solution (viscous). The solubility characteristics can vary depending on the buffer or solvent used. In appropriate buffer systems, it can be well - dispersed to carry out its enzymatic functions.​

• Biological Source: A significant source is bovine liver. However, it can also be produced through E. coli cell culture methods, which may offer advantages in terms of large - scale production and controlled conditions.​

3. Applications​

• Biochemical and Biomedical Research:​

• Since it is a key enzyme in the metabolism of glutamate, it plays a crucial role in studying nitrogen metabolism, energy production pathways related to amino acids, and the function of the tricarboxylic acid (TCA) cycle. For example, in experiments exploring how cells adapt to different nitrogen sources, the activity of this enzyme can be monitored.​

• In drug development research, it can be used as a target or a biomarker. Understanding its structure - function relationship can help in designing drugs that modulate its activity, which may be relevant for treating diseases related to abnormal amino acid metabolism, such as certain inborn errors of metabolism or neurodegenerative diseases where glutamate homeostasis is disrupted.​

• Clinical Diagnosis (Potential):​

• Although not as widely used as some other diagnostic markers, in the context of liver function assessment, changes in the activity of glutamate dehydrogenase (especially the form from bovine liver which may be used as a reference in some assays) could potentially provide additional information. Abnormal levels of this enzyme in the blood might indicate liver cell damage or dysfunction, similar to how other liver - specific enzymes are used in clinical practice. However, more research and standardization are needed to establish its routine use in clinical diagnosis.​

4. Packaging and Availability​

• Packaging Forms:​

• Available in different packaging depending on the intended use. For research applications, it can be packaged in small vials containing 25 mgm, 50 mgm, or 100 mgm quantities, which are convenient for laboratory - scale experiments.​

• When sourced from certain suppliers, it may come in lyophilized powder form in appropriate containers to maintain its stability during storage and transportation. Liquid forms may be packaged in sealed, air - tight bottles to prevent evaporation and contamination.​

• Supplier - specific Details:​

• Some suppliers offer the product with specific quality - control measures. For instance, products may be accompanied by a Certificate of Analysis (CoA) that details the enzyme's activity, purity, and other relevant parameters.