3-(N-Morpholinyl)-2-hydroxypro

Product Details

CasNo： 79803-73-9

MF： C7H14NNaO5S

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 99%

Basic Information

Product Description

Product Name：3-(N-Morpholinyl)-2-hydroxypro

CAS No: 79803-73-9

Form: Powder

Product Application

1 Protein Science

Protein Purification: Particularly suitable as a buffer for ion-exchange chromatography of proteins with isoelectric points (pI) in the 6.8-7.2 range.

Protein Crystallization: Used as a component in crystallization screens; the hydroxyl group can participate in hydrate layer formation within the crystal lattice.

Protein Stability Studies: The pH 6.9-7.1 window is critical for studying the conformation and stability of many proteins.

2 Enzymology

pH-Optima Studies: Ideal for activity assays of enzymes with optimal pH near neutrality (e.g., certain isomerases, hydrolases).

Metalloenzyme Research: Its weak metal-chelating property can be used to modulate the concentration of free metal ions in studies of metalloenzymes.

3 Cell Biology

Mitochondrial Studies: The mitochondrial matrix pH is ~7.0, making MOPSO sodium salt an excellent buffer for isolation and functional assays of mitochondria.

Specialized Microbial Culture: Suitable for culturing certain bacteria and archaea with optimal growth pH around 6.8-7.0.

4 Analytical & Separation Techniques

Capillary Electrophoresis (CE): Serves as a background electrolyte for the separation of proteins and peptides.

Isoelectric Focusing (IEF): Useful for establishing pH gradients in the 6.5-7.5 range.

Native Gel Electrophoresis: Provides conditions for separating proteins in their native conformation at near-neutral pH.

5 Biophysical Chemistry

Ligand Binding Studies: Used as a standard buffer in techniques like Isothermal Titration Calorimetry (ITC) and Surface Plasmon Resonance (SPR) for precise pH control.

pH Titration Studies: Can serve as a standard reference buffer substance.

Packaging

25Kg/Drum

Storage

Store in a tightly sealed container in a cool, dry place (2-8°C recommended). Hygroscopic.