Chinese Name: 2-((Tris(hydroxymethyl)methyl)amino)ethanesulfonic acid
Chinese Alias: N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid; Tris-ethanesulfonic acid
English Name: N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid
CAS No.: 7365-44-8
EC No.: 230-906-3
Molecular Formula: C6H15NO6S
Molecular Weight: 229.251
Physical and Chemical Properties
Appearance: White crystalline powder.
Melting Point: 225 - 228°C.
Solubility: Soluble in water, with a solubility of 50 mg/mL.
pH Range: The effective buffering range is pH 6.8 - 8.2, and the pKa value at 25°C is 7.4 or 7.5.
Quality Indicators
Assay: 99.0% - 101.0%.
Water Content: ≤1.0%.
Ultraviolet Absorbance (0.1M Aqueous Solution): ≤0.055 at 260 nm, ≤0.045 at 280 nm.
Applications
Biological Buffer: TES is an important biological buffer, suitable for various cell culture systems that require divalent metal ions. Many other buffers, such as citrates or phosphates, can cause chelation or precipitation reactions, making them unable to meet this requirement. In addition, TES is extremely beneficial for the study of succinate oxidation reactions. TES has been used in TES buffers and can also be used as a buffer in cell culture media.
Medical Detection: It can be used as a buffer in creatinine detection reagents and other medical detection fields.
Other Fields: It is also used in conductive materials. Adding TES to conductive polymers with sulfonic acid groups and/or carboxyl groups can greatly improve the conductivity and heat resistance of the resulting composition.
Storage Conditions
It can be transported and stored at room temperature, and it is stable for at least three years. The solution should be stored at 2 - 8°C and is stable for at least six months.