N-(2-Acetamido)iminodiacetic acid

Product Details

CasNo： 26239-55-4

MF： C6H10N2O5

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/bag

Purity： 99%

Basic Information

Product Description

Product Name：N-(2-Acetamido)iminodiacetic acid

CAS No: 26239-55-4

Form: Powder

Product Application

1.Biochemistry & Enzymology:

Used as a buffer for enzymatic reactions that require precise control of divalent metal ion concentrations. For example, in studies of Zn²⁺-, Mn²⁺-, or Cu²⁺-dependent enzymes​ (e.g., carbonic anhydrase, alkaline phosphatase, superoxide dismutase), ADA can buffer excess free heavy metal ions without excessively stripping the essential metal ions from the enzyme's active site.

Employed in the purification and activity assays of metalloproteases​ to maintain their stability and activity.

2.Protein Chemistry:

Used as an equilibration and elution buffer in protein purification​ (e.g., ion-exchange chromatography, affinity chromatography), particularly for proteins sensitive to heavy metal ions.

Included in protein crystallization screens; its weak chelating properties can sometimes aid in obtaining high-quality crystals.

3.Biophysical Studies:

Used in techniques like Isothermal Titration Calorimetry (ITC) and Surface Plasmon Resonance (SPR)​ to study protein-metal or protein-ligand interactions. ADA provides a clean background with controlled metal ion activity.

4.Cell Biology:

Used in media for the isolation of organelles​ (e.g., mitochondria, chloroplasts) to help maintain structural integrity and function.

Used in specific cell-free systems​ or cell extract​ experiments to maintain pH stability and control the activity of endogenous metal ions.

5.Analytical Chemistry:

Serves as an auxiliary buffer in complexometric titrations​ for the selective determination of metal ions.

Packaging

25Kg/Bag

Storage

Store the solid powder in a tightly sealed container in a cool, dry place; it is stable at room temperature. Prepared buffer solutions should be stored at 2-8°C and used within weeks to prevent microbial growth.