Solubility: Highly soluble in water, 25 g can be dissolved in 50 ml of water.
Buffer Range: pH 7.7 - 9.1
pKa Value: 8.4 (25℃)
Uses
It is an amphoteric ionic buffer widely used in the fields of biochemistry and molecular biology, and can be used as a component of Good’s buffer.
It is used as a background electrolyte for capillary zone electrophoresis in protein micro - analysis, which can maintain the pH stability of the solution, reduce protein aggregation and precipitation, and improve the separation efficiency and resolution.
It can be used as a component of the buffer solution in DNA and RNA sample analysis to protect the structure and function of nucleic acid molecules.
As a component of the culture medium in cell culture experiments, it helps to maintain the pH stability of the culture medium and provide a suitable environment for cell growth.
In the pharmaceutical industry, it is used as a buffer in the drug preparation process to control the pH value of the drug and ensure the stability and efficacy of the drug.
Storage Conditions: Store at room temperature, away from light, moisture - proof, air - tight and dry.
Safety Information: For safety and health, it is recommended to wear a lab coat and disposable gloves when using to avoid direct contact with the skin and inhalation of dust.
Synthesis Process
Usually, tris(hydroxymethyl)aminomethane (Tris) and 1,3 - propanesultone (1,3 - PS) are used as raw materials. The two raw materials are accurately weighed in molar amounts, and dissolved in a suitable amount of n - butanol solvent, placed in a three - necked flask with a reflux condenser, heated and stirred for 6 hours, and then cooled, filtered, washed, dried to obtain the product.