Solubility: Easily soluble in water, with a solubility of 1000 g/L at 20℃, and slightly soluble in organic solvents.
Buffer Range: pH 6.5 - 7.9
pKa Value: 7.2 (25℃)
Uses
Biological Buffer: Often used to prepare RNA electrophoresis buffer solutions. It is a common buffer for electrophoresis and protein purification chromatography, and can also be used to maintain the pH value of mammalian cell culture media.
Biochemical Diagnosis: Can be used in biochemical diagnosis kits, DNA/RNA extraction kits, and PCR diagnosis kits.
Other Aspects: Suitable for the study of electron transport and phosphorylation in chloroplast thylakoid preparations. Can be used as a component of the electrolyte system for isoelectric focusing electrophoresis (IEF) in two - dimensional gel electrophoresis. It is also applied in Northern blotting as a buffer for RNA separation and transfer. Besides, it can be formulated into various agar media, and used as a non - toxic buffer in the culture of Streptomyces clavuligerus and the production of cephalosporin in minimal media.
Storage Conditions: Store at room temperature, away from light and moisture.
Safety Information: It is irritating to the human body. The danger code is Xi, and the danger level is R36/37/38. When using, pay attention to protection, such as wearing a lab coat and disposable gloves to avoid direct contact with the human body or inhalation.