Solubility: Soluble in water, with a solubility of about 110 g/L at 20℃. Insoluble in most organic solvents.
Buffer Range: pH 9.7 - 11.1
pKa Value: 10.4 (at 25℃)
Flash Point: >110℃
Uses
Biochemical Diagnosis: Mainly used in biochemical diagnosis kits, DNA/RNA extraction kits, and PCR diagnosis kits. It is used as a buffer for enzymatic chemistry and HPLC separation of basic drugs.
Protein Transfer: It is a commonly used buffer in the protein transfer process, which can provide a stable alkaline environment, helping to improve the efficiency and quality of protein transfer.
Capillary Electrophoresis: Used to prepare the running buffer solution in capillary electrophoresis. As a terminating electrolyte, it can achieve electro - stacking on - line enrichment and separation of certain substances.
Other Aspects: It can support the activity of alkaline phosphatase and inhibit the growth of Aeromonas at pH 10.5. It can also be used for the purification of fibronectin. In addition, it can be used as a hydrophilic modifier to react with polyisocyanates to prepare related products.
Storage Conditions
It can be stored at room temperature. It is recommended to store it in a cool, dry and dark - place, and the temperature is preferably lower than 15℃. The shelf - life is generally 2 years.
Safety Information
It has low toxicity and good compatibility with biological molecules. However, when using it, avoid inhaling dust or direct contact with skin and eyes. In case of accidental contact, immediately rinse with a large amount of water.