N-Cyclohexyl-3-aminopropanesulfonic acid

Product Details

CasNo： 1135-40-6

MF： C9H19NO3S

Appearance： powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 99%

Basic Information

Product Description

Product Name：N-Cyclohexyl-3-aminopropanesulfonic acid

CAS No: 1135-40-6

Form: Powder

Product Application

1.Western Blotting:

Classic Transfer Buffer: CAPS buffer (e.g., 10 mM CAPS, pH 11.0, with 10% methanol) is the buffer of choice for semi-dry and wet protein transfer, especially for high-molecular-weight proteins. Its alkaline pH facilitates efficient elution of proteins from SDS-PAGE gels onto PVDF or nitrocellulose membranes.

2.Capillary Electrophoresis (CE):

Used as a background electrolyte for the separation and analysis of proteins, peptides, and basic drugs. The high pH optimizes separation by altering the charge state of analytes.

3.Chromatography & Protein Purification:

Serves as an equilibration and elution buffer for ion-exchange, hydrophobic interaction, and size-exclusion chromatography that require high-pH conditions.

4.Biocrystallography:

A component in crystallization screening kits for exploring protein crystallization conditions at alkaline pH.

5.Molecular Biology:

Useful for certain nucleic acid manipulations or reactions requiring an alkaline environment.

6.General Biochemical Assays:

Any reaction system requiring precise pH control in the range of 9.5-11.0.

Packaging

25Kg/Drum

Storage

Store in a cool, dry place in a tightly sealed container. The solid is stable at room temperature. Prepared buffer solutions should be stored at 2-8°C and used within weeks to prevent microbial contamination.