Bicine

Product Details

CasNo： 150-25-4

MF： C6H13NO4

Appearance： White crystalline powder

Delivery Time： 15 days

Packing： 25kg/drum

Purity： 99%

Basic Information

Product Description

Product Name：Bicine

CAS No: 150-25-4

Form: White crystalline powder

Product Application

1. Biochemistry & Enzymology (Core Application)

Reaction Buffer for Metal-Dependent Enzymes:​ Essential for enzymes like alkaline phosphatase, kinases, polymerases, ensuring full bioavailability of metal cofactors (Mg²⁺, Mn²⁺, Zn²⁺).

Protein Purification & Storage:​ Particularly suitable for purifying metal-sensitive proteins or as a long-term storage buffer for proteins.

Isoelectric Focusing (IEF):​ Serves as a carrier ampholyte in specific pH ranges.

2. Plant Physiology & Photosynthesis Research

Chloroplast Isolation & Activity Studies:​ A classic component of buffers for isolating intact, photosynthetically active chloroplasts because it does not interfere with the thylakoid membrane electron transport chain.

3. Cell Biology (Selected Applications)

Buffering Component in Some Serum-Free Media:​ Used in combination with a bicarbonate system to maintain stable pH in CO₂ incubators.

Organelle Isolation:​ e.g., in mitochondrial isolation buffers.

4. Electrophoresis & Chromatography

Capillary Electrophoresis (CE):​ Used as a background electrolyte.

Chromatographic Separations:​ Mobile phase for certain ion-exchange or size-exclusion chromatography.

Packaging

25Kg/Drum

Storage

Store in a tightly sealed container in a dry, cool (room temperature), dark place. The solid form is very stable. Prepared solutions should be filter-sterilized and can be stored for weeks to months at 2-8°C.