Tris Base

Product Details

CasNo： 77-86-1

MF： C4H11NO3

Appearance： White Powder

Delivery Time： 15 days

Packing： 25kg/bag

Purity： 99%

Basic Information

Product Description

Product Name：Tris(hydroxymethyl)aminomethan

CAS No: 77-86-1

Form: White crystal

Product Application

1.Molecular Biology (Core Application)

Nucleic Acid Electrophoresis Buffers:​ The core component of TAE (Tris-Acetate-EDTA)​ and TBE (Tris-Borate-EDTA)​ buffers for DNA/RNA agarose gel electrophoresis and capillary electrophoresis.

Nucleic Acid Extraction & Purification:​ Widely used in lysis, binding, wash, and elution buffers (e.g., for silica-column purification).

Hybridization & Blotting:​ Components of hybridization and wash buffers for Northern/Southern/Western blotting.

PCR & Enzymatic Reactions:​ Provides the optimal pH for enzymes like Taqpolymerase, ligases, and restriction endonucleases.

2. Protein Research

SDS-PAGE Protein Electrophoresis:​ The Tris-glycine buffer system is the global standard for denaturing polyacrylamide gel electrophoresis.

Protein Purification:​ A foundational component of equilibration, wash, and elution buffers for various chromatography techniques (ion exchange, size exclusion, affinity).

Protein Storage & Crystallization.

3. Cell Culture & Biopharmaceuticals

Cell Culture Media:​ Used as a pH adjuster and buffering agent.

Biologics Formulation:​ Maintains pH stability in vaccines, therapeutic proteins, and diagnostic reagents.

4. Other Applications

Biochemical Analysis Reagents:​ Buffer component in clinical diagnostic kits.

Surfactants & Cosmetics:​ Used as a pH adjuster.

Packaging

25Kg/Bag

Storage

Due to its strong hygroscopicity, it must be stored in a tightly sealed container in a dry, cool, dark place. After opening, it is advisable to purge with dry nitrogen or include a desiccant. Prepared solutions are stable for months at room temperature, but long-term storage at 2-8°C is recommended.