Tris Hydrochloride

Product Details

CasNo： 1185-53-1

MF： C4H12ClNO3

Appearance： White crystal

Delivery Time： 15 days

Packing： 25kg/bag

Purity： 99%

Basic Information

Product Description

Product Name：Tris Hydrochloride

CAS No: 1185-53-1

Form: White crystal

Product Application

1. Molecular Biology (Core Application)

Nucleic Acid Electrophoresis Buffers (TAE, TBE):​ Tris-HCl combined with Acetate-EDTA or Borate-EDTA is the standard buffer for DNA/RNA agarose gel electrophoresis.

Nucleic Acid Hybridization & Wash Buffers:​ e.g., for Southern/Northern blotting.

DNA/RNA Extraction & Purification:​ A component of various lysis, wash, and elution buffers.

PCR Buffer:​ Provides a stable pH environment for enzymes like TaqDNA polymerase.

2. Biochemistry & Protein Research

Protein Electrophoresis Buffers (SDS-PAGE):​ Tris-HCl/Glycine is the core buffer system for both resolving and stacking gels in protein SDS-PAGE.

Enzymatic Reaction Buffers:​ Provides the optimal pH for many enzymes (e.g., restriction endonucleases, ligases, kinases, phosphatases).

3. Cell Culture & Biologics

Cell Culture Media Additive:​ Used to adjust and stabilize the pH of media.

Pharmaceutical Formulations:​ Acts as a pH stabilizer in some injectables, vaccines, and diagnostic reagents.

4. Other Applications

Diagnostic Reagents:​ A component of calibrators and reagents for biochemical analyzers.

Cosmetics:​ Used as a pH adjuster.

Packaging

25Kg/Bag

Storage

Store in a tightly sealed container in a dry, cool , and dark place. Prepared solutions are stable for months at room temperature; for long-term storage, 2-8°C is recommended.